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Southeast Asian J Trop Med Public Health ; 2002 ; 33 Suppl 3(): 45-8
Article in English | IMSEAR | ID: sea-35224

ABSTRACT

In Thailand in 1999-2000, Cyclospora oocysts from two HIV-infected patients and one patient with prolonged diarrhea were detected by formalin-ether concentration technique. Sporulation was performed by mixing stool samples in 2.5% potassium dichromate solution, sporulated oocysts were then treated with various solutions before mechanical rupturing in order to establish excystation, fewer than 10% of the sporulated oocysts could be excysted. Our techniques provided more details of the characteristic appearance of sporocysts and sporozoites within the oocysts (DMSO-modified acid-fast technique with our modification).


Subject(s)
Animals , Cell Line , Cyclospora/growth & development , Dogs , HIV Infections/psychology , Humans , Thailand
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